{"id":544,"date":"2025-02-15T10:43:34","date_gmt":"2025-02-15T10:43:34","guid":{"rendered":"https:\/\/rhirota.hiroshima-u.ac.jp\/?page_id=544"},"modified":"2025-02-27T04:58:10","modified_gmt":"2025-02-27T04:58:10","slug":"publication","status":"publish","type":"page","link":"https:\/\/rhirota.hiroshima-u.ac.jp\/?page_id=544","title":{"rendered":"Publication"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"544\" class=\"elementor elementor-544\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1e6f436 e-flex e-con-boxed e-con e-parent\" data-id=\"1e6f436\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-aaf129c elementor-widget elementor-widget-spacer\" data-id=\"aaf129c\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-e4b8b1e e-flex e-con-boxed e-con e-parent\" data-id=\"e4b8b1e\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-00a3dcb elementor-widget elementor-widget-heading\" data-id=\"00a3dcb\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Publication<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-87c4e0c elementor-widget elementor-widget-heading\" data-id=\"87c4e0c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u5b66\u8853\u8ad6\u6587 \u8457\u66f8 \u7279\u8a31\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c2e411c e-flex e-con-boxed e-con e-parent\" data-id=\"c2e411c\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-0d29a79 e-con-full e-flex e-con e-child\" data-id=\"0d29a79\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-67f17f5 e-con-full e-flex e-con e-child\" data-id=\"67f17f5\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c3ef835 elementor-widget elementor-widget-heading\" data-id=\"c3ef835\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u5b66\u8853\u8ad6\u6587<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-dcaa464 e-con-full e-flex e-con e-child\" data-id=\"dcaa464\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-9f8430f elementor-widget elementor-widget-text-editor\" data-id=\"9f8430f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ol><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Toda N, Inoue-Kashino N, Fujita H, Yoshida R, Nimura-Matsune K, Watanabe S, Kuroda A, Kashino Y, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">. Cell morphology engineering enhances grazing resistance of <\/span><i><span style=\"font-weight: 400;\">Synechococcus elongatu<\/span><\/i><span style=\"font-weight: 400;\"><span style=\"font-weight: 400;\">s PCC 7942 for non-sterile large-scale cultivation. J Biosci Bioeng, 137(4):245-253, 2024.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Asih D, Handayani W, Ananda A, Yuniati R, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\"><span style=\"font-weight: 400;\">, Summerfield T, Eaton-Rye J. An insight into potential phosphate bioremediation and renewable energy from agricultural waste via integrated wastewater treatment systems in Indonesia. Environ Res Commun, 6:042001, 2024.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Abdelhady GN, Tajima T, Ikeda T, Ishida T, Funabashi H, Kuroda A, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">. A novel salt- and organic solvent-tolerant phosphite dehydrogenase from <\/span><i><span style=\"font-weight: 400;\">Cyanothece<\/span><\/i><span style=\"font-weight: 400;\"><span style=\"font-weight: 400;\"> sp. ATCC 51142. Front Bioeng Biotechnol, 11:1255582, 2023.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Kobayashi I, Imamura S, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kuroda A, Tanaka K. Expression of bacterial phosphite dehydrogenase confers phosphite availability in a unicellular red alga <\/span><i><span style=\"font-weight: 400;\">Cyanidioschyzon merolae<\/span><\/i><span style=\"font-weight: 400;\"><span style=\"font-weight: 400;\">. The Journal of General and Applied Microbiology, 69:287\u2013291, 2023.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Nishimura T, Ishida T, Funabashi H, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\"><span style=\"font-weight: 400;\">, Ikeda T, Kuroda A: Detection of Fine Asbestos Fibers Using Fluorescently Labeled Asbestos-Binding Proteins in Talc. Journal of Hazardous Materials Advances, 12:1-8, 2023.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Bown L, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\"><span style=\"font-weight: 400;\">, Goettge M, Cui J, Krist D, Zhu L, Giurgiu C, Van der Donk W, Ju K-S, Metcalf W: A novel pathway for biosynthesis of the herbicidal phosphonate natural product phosphonothrixin is widespread in actinobacteria. J Bacteriol, 205(5): e00485-22, 2023.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Masaki K, Ahmed ABF, Ishida T, Funabashi H, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Ikeda T, Kuroda A: Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes. Biotechnol Lett, 45:1457\u20131466, 2023.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Murakami H, Sano K, Motomura K, Kuroda A, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">: Assessment of horizontal gene transfer-mediated destabilization of <\/span><i><span style=\"font-weight: 400;\">Synechococcus elongatus<\/span><\/i><span style=\"font-weight: 400;\"> PCC 7942 biocontainment system. J Biosci Bioeng, 135(3):190-195, 2023.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Katsuura Z, Momokawa N, Murakami H, Watanabe S, Ishida T, Ikeda T, Funabashi H, Kuroda A: Gatekeeper residue replacement in a phosphite transporter enhances mutational robustness of the biocontainment strategy. ACS Synth Biol, 11(10): 3397-3404, 2022.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Inoue H, Tajima K, Mitsumori C, Inoue-Kashino N, Miura T, Ifuku K, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kashino Y, Fujita K, Kinoshita H: Biodiversity risk assessment of genetically modified <\/span><i><span style=\"font-weight: 400;\">Chaetoceros gracilis<\/span><\/i><span style=\"font-weight: 400;\"> for outdoor cultivation. J Gen Appl Microbiol, 68(3):151-162, 2022.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Funabashi H, Nakatsuka K, Yoshida S, Shigeto H, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Ikeda T, Kuroda A: Design of split G-quadruplex-based DNA\u2013bridged nucleic acid chimera nano tweezers that recognize short nucleic acids with a single-base mismatch. Sens Mater, 34:2022, 2022.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Toda N, Murakami H, Kanbara A, Kuroda A, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">: Phosphite reduces the predation impact of <\/span><i><span style=\"font-weight: 400;\">Poterioochromonas malhamensis<\/span><\/i><span style=\"font-weight: 400;\"> on cyanobacterial culture. Plants, 10(7):1361, 2021.\u3000(Feature Paper)<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Abdelhady GN, Ikeda T, Ishida T, Funabashi H, Kuroda A, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">: Engineering cofactor specificity of a thermostable phosphite dehydrogenase for a highly efficient and robust NADPH regeneration system. Front Bioeng Biotechnol,9:647176, 2021.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ishida T, Hashimoto T, Masaki K, Funabashi H, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Ikeda T, Tajima H, Kuroda A: Application of peptides with an affinity for phospholipid membranes during the automated purification of extracellular vesicles. Sci Rep, 10(1):18718, 2020.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ikeda T, Ichikawa K, Shigeto H, Ishida T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Funabashi H, Kuroda A: Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish. Biosci Biotechnol Biochem, 83(12): 2272-2275, 2019.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ishida T, Fujihara N, Nishimura T, Funabashi H, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Ikeda T, Kuroda A: Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy. Genes Environ, 41:1-11, 2019.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Shigeto H, Ono T, Ikeda T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Ishida T, Kuroda A, Funabashi H: Insulin sensor cells for the analysis of insulin secretion responses in single living pancreatic <\/span><span style=\"font-weight: 400;\">\u03b2<\/span><span style=\"font-weight: 400;\"> cells. Analyst, 144(12): 3765-3772, 2019.<\/span><\/span><\/span><\/span><p>\u00a0<\/p><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Motomura K, Sano K, Watanabe S, Kanbara A, Abdelhady GN, Ikeda T, Ishida T, Funabashi H, Kuroda A and <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">: Synthetic phosphorus metabolic pathway for biosafety and contamination management of cyanobacterial cultivation. ACS Synth Biol, 7(9): 2189-2198, 2018.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Abe K, Katsuura Z, Noguchi R, Moribe S, Motomura K, Ishida T, Alexandrov M, Funabashi H, Ikeda T and Kuroda A: A novel biocontainment strategy makes bacterial growth and survival dependent on phosphite. Sci Rep, 7(1): 1-10, 2017.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Shigeto H, Nakatsuka K, Ikeda T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kuroda A and Funabashi H: Continuous monitoring of specific mRNA expression responses with a fluorescence resonance energy transfer-based DNA nano-tweezer technique that does not require gene recombination. Anal Chem, 88 (16), 7894-7898, 2016.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Saiki A, Ishida Y, Segawa S, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Nakamura T, Kuroda A: A <\/span><i><span style=\"font-weight: 400;\">Lactobacillus<\/span><\/i><span style=\"font-weight: 400;\"> mutant capable of accumulating long-chain polyphosphates that enhance intestinal barrier function. Biosci Biotechnol Biochem, 80: 955-961, 2016<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Motomura K, Ikeda T, Matsuyama S, Abdelhamid MA, Tanaka T, Ishida T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kuroda A: The C-terminal zwitterionic sequence of CotB1 is essential for biosilicification of the <\/span><i><span style=\"font-weight: 400;\">Bacillus cereus<\/span><\/i><span style=\"font-weight: 400;\"> spore coat. J Bacteriol, 198: 276-282, 2016.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Abdelhamid MA, Ikeda T, Motomura K, Tanaka T, Ishida T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kuroda A: Application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag. J Biosci Bioeng, 122(5):633-638, 2016.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Kuroda A and <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">: Environmental biotechnology for efficient utilization of industrial phosphite waste. Global Environ. Res., 19(1), 77-82, 2015.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ju KS, Gao J, Doroghazi JR, Wang KK, Thibodeaux CJ, Li S, Metzger E, Fudala J, Su J, Zhang JK, Lee J, Cioni JP, Evans BS, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Labeda DP, van der Donk WA, Metcalf WW: Discovery of phosphonic acid natural products by mining the genomes of 10,000 actinomycetes. Proc Natl Acad Sci U S A, 112: 12175-12180, 2015.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Alexandrov M, Ichida E, Nishimura T, Aoki K, Ishida T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Ikeda T, Kawasaki T, Kuroda A: Development of an automated asbestos counting software based on fluorescence microscopy. Environ Monit Assess, 187: 4166, 2015.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Motomura K, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Okada M, Ikeda T, Ishida T, Kuroda A: A new subfamily of polyphosphate kinase 2 (class III PPK2) catalyzes both nucleoside monophosphate phosphorylation and nucleoside diphosphate phosphorylation. Appl Environ Microbiol, 80: 2602-2608, 2014.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Kanda K, Ishida T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Ono S, Motomura K, Ikeda T, Kitamura K, Kuroda A: Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts. J Biotechnol, 182-183: 68-73, 2014.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Abdelhamid MA, Motomura K, Ikeda T, Ishida T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kuroda A: Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag. Appl Microbiol Biotechnol, 98: 5677-5684, 2014.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ishida T, Alexandrov M, Nishimura T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Ikeda T and Kuroda A: Molecular engineering of a fluorescent bioprobe for sensitive and selective detection of amphibole asbestos. PLoS One, 8: e76231, 2013.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Motomura K, Nakai S, Handa T, Ikeda T and Kuroda A: Stable polyphosphate accumulation by a pseudo-revertant of an <\/span><i><span style=\"font-weight: 400;\">Escherichia coli phoU<\/span><\/i><span style=\"font-weight: 400;\"> mutant. Biotechnol Lett, 35: 695-701, 2013.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ye X, Honda K, Sakai T, Okano K, Omasa T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kuroda A and Ohtake H: Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway. Microb Cell Fact, 11: 120, 2012.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Restiawaty E, Honda K, Okano K, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Omasa T, Kuroda A and Ohtake H: Construction of membrane-anchoring fusion protein of <\/span><i><span style=\"font-weight: 400;\">Thermococcus kodakaraensis<\/span><\/i><span style=\"font-weight: 400;\"> glycerol kinase and its application to repetitive batchwise reactions. J Biosci Bioeng, 113: 521-525, 2012.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ishida T, Alexandrov M, Nishimura T, Minakawa K, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Sekiguchi K, Kohyama N and Kuroda A: Evaluation of sensitivity of fluorescence-based asbestos detection by correlative microscopy. J Fluoresc, 22: 357-363, 2012.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Yamane ST, Fujibuchi T, Motomura K, Ishida T, Ikeda T and Kuroda A: Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from <\/span><i><span style=\"font-weight: 400;\">Ralstonia<\/span><\/i><span style=\"font-weight: 400;\"> sp. strain 4506. J Biosci Bioeng, 113: 445-450, 2012.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Motomura K, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Ohnaka N, Okada M, Ikeda T, Morohoshi T, Ohtake H and Kuroda A: Overproduction of YjbB reduces the level of polyphosphate in <\/span><i><span style=\"font-weight: 400;\">Escherichia coli<\/span><\/i><span style=\"font-weight: 400;\">: a hypothetical role of YjbB in phosphate export and polyphosphate accumulation. FEMS Microbiol Lett, 320: 25-32, 2011.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ikeda T, Motomura K, Agou Y, Ishida T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\"> and Kuroda A: The silica-binding Si-tag functions as an affinity tag even under denaturing conditions. Protein Expr Purif, 77: 173-177, 2011.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ito I, Hanyu A, Wayama M, Goto N, Katsuno Y, Kawasaki S, Nakajima Y, Kajiro M, Komatsu Y, Fujimura A, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Murayama A, Kimura K, Imamura T and Yanagisawa J: Estrogen inhibits transforming growth factor beta signaling by promoting Smad2\/3 degradation. J Biol Chem, 285: 14747-14755, 2010.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ishida T, Alexandrov M, Nishimura T, Minakawa K, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Sekiguchi K, Kohyama N and Kuroda A: Selective detection of airborne asbestos fibers using protein-based fluorescent probes. Environ Sci Technol, 44: 755-759, 2010.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ikeda T, Ninomiya K, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\"> and Kuroda A: Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner. Protein Expr Purif, 71: 91-95, 2010.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Honda K, Maya S, Omasa T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kuroda A and Ohtake H: Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes. J Biotechnol, 148: 204-207, 2010.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Hata Y, Ikeda T, Ishida T and Kuroda A: The silicon layer supports acid resistance of <\/span><i><span style=\"font-weight: 400;\">Bacillus cereus<\/span><\/i><span style=\"font-weight: 400;\"> spores. J Bacteriol, 192: 111-116, 2010.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Satoh T, Tsuruta K, Shinoda Y, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Noda K, Kuroda A and Murakami Y: Reciprocating-flow ATP amplification system for increasing the number of amplification cycles. Anal Biochem, 395: 161-165, 2009.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Satoh T, Shinoda Y, Tokonami S, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Noda K, Kuroda A and Murakami Y: Continuous-flow ATP amplification system on a chip Sens. Actuators B, <\/span><i><span style=\"font-weight: 400;\">142<\/span><\/i><span style=\"font-weight: 400;\">, 118-122,2009.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Kajiro M, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\"> (co-first author), Nakajima Y, Kawanowa K, So-ma K, Ito I, Yamaguchi Y, Ohie SH, Kobayashi Y, Seino Y, Kawano M, Kawabe Y, Takei H, Hayashi S, Kurosumi M, Murayama A, Kimura K and Yanagisawa J: The ubiquitin ligase CHIP acts as an upstream regulator of oncogenic pathways. Nat Cell Biol, 11: 312-319, 2009.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Ikeda T, Hata Y, Ninomiya K, Ikura Y, Takeguchi K, Aoyagi S, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\"> and Kuroda A: Oriented immobilization of antibodies on a silicon wafer using Si-tagged protein A. Anal Biochem, 385: 132-137, 2009.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Motoi N, Suzuki K, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Johnson P, Oofusa K, Kikuchi Y and Yoshizato K: Identification and characterization of nucleoplasmin 3 as a histone-binding protein in embryonic stem cells. Dev Growth Differ, 50: 307-320, 2008.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Kuroda A, Nishimura T, Ishida T, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\"> and Nomura K: Detection of chrysotile asbestos by using a chrysotile-binding protein. Biotechnol Bioeng, 99: 285-289, 2008.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Iwamoto S, Motomura K, Shinoda Y, Urata M, Kato J, Takiguchi N, Ohtake H, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\"> and Kuroda A: Use of an <\/span><i><span style=\"font-weight: 400;\">Escherichia coli<\/span><\/i><span style=\"font-weight: 400;\"> recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate. Appl Environ Microbiol, 73: 5676-5678, 2007.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Tateishi Y, Sonoo R, Sekiya Y, Sunahara N, Kawano M, Wayama M, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kawabe Y, Murayama A, Kato S, Kimura K and Yanagisawa J: Turning off estrogen receptor beta-mediated transcription requires estrogen-dependent receptor proteolysis. Mol Cell Biol, 26: 7966-7976, 2006.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kuroda A, Ikeda T, Takiguchi N, Ohtake H and Kato J: Transcriptional analysis of the multicopy <\/span><i><span style=\"font-weight: 400;\">hao<\/span><\/i><span style=\"font-weight: 400;\"> gene coding for hydroxylamine oxidoreductase in <\/span><i><span style=\"font-weight: 400;\">Nitrosomonas<\/span><\/i><span style=\"font-weight: 400;\"> sp. strain ENI-11. Biosci Biotechnol Biochem, 70: 1875-1881, 2006.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kato J, Morita H, Kuroda A, Ikeda T, Takiguchi N and Ohtake H: Isolation and characterization of <\/span><i><span style=\"font-weight: 400;\">cbbL<\/span><\/i><span style=\"font-weight: 400;\"> and <\/span><i><span style=\"font-weight: 400;\">cbbS<\/span><\/i><span style=\"font-weight: 400;\"> genes encoding form I ribulose-1,5-bisphosphate carboxylase\/oxygenase large and small subunits in <\/span><i><span style=\"font-weight: 400;\">Nitrosomonas<\/span><\/i><span style=\"font-weight: 400;\"> sp. strain ENI-11. Biosci Biotechnol Biochem, 66: 632-635, 2002.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kato J, Kuroda A, Ikeda T, Takiguchi N, Ohtake H: Molecular biology of ammonia-oxidizing bacteria. J. Environ. Biotechnol, 2: 135-143, 2002.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Yamagata A, <\/span><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Kato J, Kuroda A, Ikeda T, Takiguchi N and Ohtake H: Mutational analysis of the multicopy <\/span><i><span style=\"font-weight: 400;\">hao<\/span><\/i><span style=\"font-weight: 400;\"> gene coding for hydroxylamine oxidoreductase in <\/span><i><span style=\"font-weight: 400;\">Nitrosomonas<\/span><\/i><span style=\"font-weight: 400;\"> sp. strain ENI-11. Biosci Biotechnol Biochem, 64: 1754-1757, 2000.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R<\/span><span style=\"font-weight: 400;\">, Yamagata A, Kato J, Kuroda A, Ikeda T, Takiguchi N and Ohtake H: Physical map location of the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium <\/span><i><span style=\"font-weight: 400;\">Nitrosomonas<\/span><\/i><span style=\"font-weight: 400;\"> sp. strain ENI-11. J Bacteriol, 182: 825-828, 2000.<br \/><br \/><\/span><\/span><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\">Yamagata A, Kato J, <span style=\"font-style: inherit; text-align: var(--text-align); background-color: var(--ast-global-color-5);\">Hirota R<\/span><span style=\"font-style: inherit; text-align: var(--text-align); background-color: var(--ast-global-color-5);\">, Kuroda A, Ikeda T, Takiguchi N and Ohtake H: Isolation and characterization of two cryptic plasmids in the ammonia-oxidizing bacterium <\/span><i style=\"font-weight: inherit; text-align: var(--text-align); background-color: var(--ast-global-color-5);\">Nitrosomonas<\/i><span style=\"font-style: inherit; text-align: var(--text-align); background-color: var(--ast-global-color-5);\"> sp. strain ENI-11. J Bacteriol, 181: 3375-81, 1999.<\/span><\/span><\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-fb738d8 e-flex e-con-boxed e-con e-parent\" data-id=\"fb738d8\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-712e6a6 e-con-full e-flex e-con e-child\" data-id=\"712e6a6\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-5b57b7a e-con-full e-flex e-con e-child\" data-id=\"5b57b7a\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7ceaa6a elementor-widget elementor-widget-heading\" data-id=\"7ceaa6a\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u8457\u66f8<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-6498de8 e-con-full e-flex e-con e-child\" data-id=\"6498de8\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-9e0f9f0 elementor-widget elementor-widget-text-editor\" data-id=\"9e0f9f0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ol><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\uff1a\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u5fae\u751f\u7269\u306e\u30ab\u30eb\u30bf\u30d8\u30ca\u7b2c\u4e00\u7a2e\u4f7f\u7528\u306b\u5411\u3051\u305f\u6280\u8853\u958b\u767a\u52d5\u5411\uff0c\u30d0\u30a4\u30aa\u30b5\u30a4\u30a8\u30f3\u30b9\u3068\u30a4\u30f3\u30c0\u30b9\u30c8\u30ea\u30fc\uff08\u4e00\u822c\u8ca1\u56e3\u6cd5\u4eba\u30d0\u30a4\u30aa\u30a4\u30f3\u30c0\u30b9\u30c8\u30ea\u30fc\u5354\u4f1a\uff09\uff1a81(4)\uff0c368-372\uff0c2023.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\uff1a\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u5fae\u751f\u7269\u306e\u5c4b\u5916\u5229\u7528\u3068\u5b89\u5168\u6027\u5411\u4e0a\u306b\u95a2\u3059\u308b\u65b9\u7b56, \u30bd\u30d5\u30c8\u30fb\u30c9\u30ea\u30f3\u30af\u6280\u8853\u8cc7\u6599\uff08\u4e00\u822c\u793e\u56e3\u6cd5\u4eba \u5168\u56fd\u6e05\u6dbc\u98f2\u6599\u9023\u5408\u4f1a\uff09\uff1a193(1), 99-111, 2021.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\uff1a\u4e9c\u30ea\u30f3\u9178\u5ec3\u68c4\u7269\u306e\u5b9f\u969b\uff0c\u30ea\u30f3\u3092\u3081\u3050\u308b\u30ec\u30dd\u30fc\u30c8\uff08\u4e00\u822c\u793e\u56e3\u6cd5\u4eba\u30ea\u30f3\u5faa\u74b0\u7523\u696d\u632f\u8208\u6a5f\u69cb\uff09\uff1a1-3, 2020.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R, Motomura K and Kuroda A: Biological phosphite oxidation and its application to phosphorus recycling. Phosphorus Recovery and Recycling, 499-514, 2019.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\uff1a\u30ea\u30f3\u4ee3\u8b1d\u7d4c\u8def\u306e\u30c7\u30b6\u30a4\u30f3\u306b\u3088\u308b\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u751f\u7269\u306e\u30d0\u30a4\u30aa\u30bb\u30fc\u30d5\u30c6\u30a3\u6280\u8853\u958b\u767a\uff0c\u30d0\u30a4\u30aa\u30b5\u30a4\u30a8\u30f3\u30b9\u3068\u30a4\u30f3\u30c0\u30b9\u30c8\u30ea\u30fc\uff08\u4e00\u822c\u8ca1\u56e3\u6cd5\u4eba\u30d0\u30a4\u30aa\u30a4\u30f3\u30c0\u30b9\u30c8\u30ea\u30fc\u5354\u4f1a\uff09\uff1a77(2)\uff0c144-145\uff0c2018.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\uff1a\u30ea\u30f3\u4ee3\u8b1d\u7d4c\u8def\u306e\u518d\u69cb\u7bc9\u306b\u3088\u308b\u30d0\u30a4\u30aa\u30bb\u30fc\u30d5\u30c6\u30a3\u6280\u8853\u958b\u767a, \u5e83\u5cf6\u5927\u5b66\u81ea\u7136\u79d1\u5b66\u7814\u7a76\u652f\u63f4\u958b\u767a\u30bb\u30f3\u30bf\u30fc\u5e74\u5831\uff08\u5e83\u5cf6\u5927\u5b66\u81ea\u7136\u79d1\u5b66\u7814\u7a76\u652f\u63f4\u958b\u767a\u30bb\u30f3\u30bf\u30fc\uff09, 116, 2018.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00<\/span><span style=\"font-weight: 400;\">:<\/span><span style=\"font-weight: 400;\"><span style=\"font-weight: 400;\"> \u4e9c\u30ea\u30f3\u9178\u306e\u5229\u7528\u3001\u30db\u30b9\u30db\u30f3\u9178\u3001\u30ea\u30f3\u306e\u8f38\u9001\u3068\u8caf\u8535\uff0c\u30ea\u30f3\u306e\u8f9e\u5178\uff08\u671d\u5009\u66f8\u5e97\uff09\uff1app84, 86, 100, 2018.<\/span><\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b\uff1a\u4e9c\u30ea\u30f3\u9178\u30c7\u30d2\u30c9\u30ed\u30b2\u30ca\u30fc\u30bc\u3092\u5229\u7528\u3057\u305f\u5fae\u751f\u7269\u306e\u9078\u629e\u7684\u57f9\u990a\u6280\u8853\uff0c\u5316\u5b66\u3068\u751f\u7269\uff08\u65e5\u672c\u8fb2\u82b8\u5316\u5b66\u4f1a\uff09\uff1a55(6), 369-371, 2017.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b\uff1a\u4e9c\u30ea\u30f3\u9178\u3092\u5229\u7528\u3057\u305f\u5b9f\u7528\u7684\u306a\u751f\u7269\u5b66\u7684\u5c01\u3058\u8fbc\u3081\u624b\u6cd5, \u30b1\u30df\u30ab\u30eb\u30a8\u30f3\u30b8\u30cb\u30e4\u30ea\u30f3\u30b0\uff08\u5316\u5b66\u5de5\u696d\u793e\uff09\uff1a62(9), 28-34, 2017.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b\uff1a\u4e9c\u30ea\u30f3\u9178\u3092\u5229\u7528\u3057\u305f\u30ed\u30d0\u30b9\u30c8\u306a\u5fae\u751f\u7269\u306e\u57f9\u990a\u3068\u751f\u7269\u5b66\u7684\u5c01\u3058\u8fbc\u3081\uff0c\u5316\u5b66\u5de5\u696d\uff08\u5316\u5b66\u5de5\u696d\u793e\uff09\uff1a68(6), 41-47, 2017.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u52a0\u85e4\u7d14\u4e00\u3001\u7530\u5cf6\u8a89\u4e45\u3001\u9ed2\u7530\u7ae0\u592b\u3001\u5ee3\u7530\u9686\u4e00\u3001\u672c\u7530\u5b5d\u4ecb\u3001\u5927\u7af9\u4e45\u592b: \u4f4e\u70ad\u7d20\u30b1\u30df\u30ab\u30eb\u751f\u7523\u306e\u305f\u3081\u306e\u30b7\u30f3\u30d7\u30eb\u9175\u7d20\u89e6\u5a92, \u30b1\u30df\u30ab\u30eb\u30a8\u30f3\u30b8\u30cb\u30e4\u30ea\u30f3\u30b0\uff08\u5316\u5b66\u5de5\u696d\u793e\uff09: 61(3), pp1-5,2016.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b: \u9084\u5143\u578b\u30ea\u30f3\u5316\u5408\u7269\u3092\u5229\u7528\u3057\u305f\u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc, \u9175\u7d20\u5de5\u5b66\u30cb\u30e5\u30fc\u30b9\uff08\u9175\u7d20\u5de5\u5b66\u7814\u7a76\u4f1a\uff09: 74, pp26-31, 2015.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b: \u30d0\u30a4\u30aa\u5909\u63db\u306b\u3088\u308b\u8cb4\u91cd\u30ea\u30f3\u8cc7\u6e90\u306e\u56de\u53ce\u30fb\u6709\u52b9\u5229\u7528\u6280\u8853, \u30d0\u30a4\u30aa\u30d9\u30fc\u30b9\u8cc7\u6e90\u78ba\u4fdd\u6226\u7565\uff08CMC\u51fa\u7248\uff09: pp135-143, 2015.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b: \u8010\u71b1\u6027\u4e9c\u30ea\u30f3\u9178\u30c7\u30d2\u30c9\u30ed\u30b2\u30ca\u30fc\u30bc\u306e\u767a\u898b\u3068\u5fdc\u7528, \u74b0\u5883\u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u5b66\u4f1a\u8a8c, 14(1): 15-20, 2014.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b: \u6392\u6c34\u304b\u3089\u306e\u30ea\u30f3\u8cc7\u6e90\u306e\u56de\u53ce\u2212\u57fa\u790e\u2212\u5fae\u751f\u7269\u306b\u3088\u308b\u30ea\u30f3\u8cc7\u6e90\u306e\u52b9\u7387\u7684\u56de\u53ce\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50 \u30d0\u30a4\u30aa\u6d3b\u7528\u306b\u3088\u308b\u6c5a\u67d3\u30fb\u5ec3\u6c34\u306e\u65b0\u51e6\u7406\u6cd5\uff08CMC\u51fa\u7248\uff09: pp66-75, 2012.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u9ed2\u7530\u7ae0\u592b\u3001\u5ee3\u7530\u9686\u4e00: \u30ea\u30f3\u9178\u306e\u7121\u6a5f\u5316\u5b66\u3068\u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\uff08\u751f\u7269\u5de5\u5b66\u4f1a\u8a8c\uff09, 90(8), pp473-476, 2012.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b: \u8010\u71b1\u6027\u9175\u7d20\u3092\u7528\u3044\u305f\u30d0\u30a4\u30aa\u30d7\u30ed\u30bb\u30b9\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u306e\u958b\u767a, \u74b0\u5883\u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u5b66\u4f1a\u8a8c, 11(1\uff652): 54-60, 2011.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\u3001\u6c60\u7530\u4e08\u3001\u9ed2\u7530\u7ae0\u592b: \u30d0\u30af\u30c6\u30ea\u30a2\u306b\u304a\u3051\u308b\u30b7\u30ea\u30b3\u30f3\u306e\u5f79\u5272, \u74b0\u5883\u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u5b66\u4f1a\u8a8c, 11(1\uff652): 47-53, 2011.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u9ed2\u7530\u7ae0\u592b\u3001\u5ee3\u7530\u9686\u4e00\u3001\u77f3\u7530\u4e08\u5178\u3001\u6c60\u7530\u4e08: \u30d0\u30af\u30c6\u30ea\u30a2\u306e\u30b7\u30ea\u30b3\u30f3\u30d0\u30a4\u30aa\u30b5\u30a4\u30a8\u30f3\u30b9\uff08\u5316\u5b66\u3068\u751f\u7269\uff09: 49(6), pp377-384, 2011.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">Hirota R, Kuroda A, Kato J and Ohtake H: Bacterial phosphate metabolism and its application to phosphorus recovery and industrial bioprocesses. J Biosci Bioeng, 109: 423-32, 2010.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u9ed2\u7530\u7ae0\u592b\u3001\u5ee3\u7530\u9686\u4e00\u3001\u672c\u6751\u572d: \u5fae\u751f\u7269\u306e\u30dd\u30ea\u30ea\u30f3\u9178\u84c4\u7a4d\u6a5f\u69cb\u89e3\u660e\u3068\u5229\u7528\uff08Phosphorus Letter\uff09: 68, pp7-18, 2010.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u9ed2\u7530\u7ae0\u592b\u3001\u5ee3\u7530\u9686\u4e00\u3001\u672c\u6751\u572d: \u5fae\u751f\u7269\u306e\u30dd\u30ea\u30ea\u30f3\u9178\u84c4\u7a4d\u6a5f\u69cb\u89e3\u660e\u3068\u30ea\u30f3\u6fc3\u7e2e\u3078\u306e\u5229\u7528, \u30ea\u30f3\u8cc7\u6e90\u306e\u56de\u53ce\u3068\u6709\u52b9\u5229\u7528\uff08\u30b5\u30a4\u30a8\u30f3\u30b9\uff06\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\uff09: pp63-75, 2009.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u5ee3\u7530\u9686\u4e00\uff1a\u74b0\u5883\u554f\u984c\u306e\u672c\u8cea\u3092\u8003\u3048\u308b\uff08\u751f\u7269\u5de5\u5b66\u4f1a\u8a8c\uff09: 87, pp86, 2009.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\"><span style=\"font-weight: 400;\">\u9ed2\u7530\u7ae0\u592b\u3001\u6751\u4e0a\u88d5\u4e8c\u3001\u91ce\u7530\u5065\u4e00\u3001\u5ee3\u7530\u9686\u4e00: ATP\u30d0\u30a4\u30aa\u30bb\u30f3\u30b7\u30f3\u30b0\u7d20\u5b50\u958b\u767a\u3068\u30c1\u30c3\u30d7\u5316\uff08\u751f\u7269\u5de5\u5b66\u4f1a\u8a8c\uff09\uff1a 86, pp274-276, 2008.<\/span><\/span><p>\u00a0<\/p><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b: \u8010\u71b1\u6027\u30dd\u30ea\u30ea\u30f3\u9178\u30ad\u30ca\u30fc\u30bc\u3092\u7528\u3044\u305fATP\u518d\u751f\u7cfb\u3068\u7269\u8cea\u751f\u7523\uff0c\u30d0\u30a4\u30aa\u30b5\u30a4\u30a8\u30f3\u30b9\u3068\u30a4\u30f3\u30c0\u30b9\u30c8\u30ea\u30fc\uff08\u4e00\u822c\u8ca1\u56e3\u6cd5\u4eba\u30d0\u30a4\u30aa\u30a4\u30f3\u30c0\u30b9\u30c8\u30ea\u30fc\u5354\u4f1a\uff09: 25, pp21-28, 2008.<\/span><\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-b008fa4 e-flex e-con-boxed e-con e-parent\" data-id=\"b008fa4\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-f38a689 e-con-full e-flex e-con e-child\" data-id=\"f38a689\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-d784172 e-con-full e-flex e-con e-child\" data-id=\"d784172\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-a7e7270 elementor-widget elementor-widget-heading\" data-id=\"a7e7270\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u7279\u8a31<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-aec3cfb e-con-full e-flex e-con e-child\" data-id=\"aec3cfb\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1a85182 elementor-widget elementor-widget-text-editor\" data-id=\"1a85182\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ol><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b\u3001\u4e09\u8f2a\u6731\u8389\u3001\u767e\u5ddd\u76f4\u8f1d\uff1a\u30ea\u30f3\u5316\u5408\u7269\u3092\u7528\u3044\u305f\u5fae\u751f\u7269\u306e\u5897\u6b96\u5236\u5fa1\u65b9\u6cd5\u3001\u7279\u98582024-124810<br \/><br \/><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b\u3001\u30ac\u30de\u30eb\u30fb\u30ca\u30c3\u30b5\u30fc\u30fb\u30a2\u30d6\u30c7\u30eb\u30cf\u30c7\u30a3: \u65b0\u898f\u306a\u4e9c\u30ea\u30f3\u9178\u30c7\u30d2\u30c9\u30ed\u30b2\u30ca\u30fc\u30bc\u304a\u3088\u305d\u306e\u5229\u7528\uff0c\u7279\u98582023-022904<br \/><br \/><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b: \u85cd\u85fb\u306e\u5909\u7570\u4f53\u304a\u3088\u3073\u305d\u306e\u88fd\u9020\u65b9\u6cd5, \u7279\u98582022-158914<br \/><br \/><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b\u3001\u83d3\u5b50\u91ce\u5eb7\u6d69\u3001\u83d3\u5b50\u91ce\u540d\u6d25\u5b50\u3001\u6e21\u8fba\u667a: \u85cd\u85fb\u306e\u57f9\u990a\u65b9\u6cd5\u304a\u3088\u3073\u85cd\u85fb\u306e\u5909\u7570\u682a\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u65b9\u6cd5\uff0c\u7279\u98582021-148599<br \/><br \/><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\">\u5ee3\u7530\u9686\u4e00\u3001\u9ed2\u7530\u7ae0\u592b: \u5f62\u8cea\u8ee2\u63db\u4f53\u306e\u5897\u6b96\u5236\u5fa1\u6cd5, \u7279\u98582020-26368<br \/><br \/><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 10pt;\">\u5ee3\u7530\u9686\u4e00\u3001\u672c\u6751\u572d\u3001\u9ed2\u7530\u7ae0\u592b: 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